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A Cre-dependent, anterograde transsynaptic viral tracer for mappi…
https://pubmed.ncbi.nlm.nih.gov/22196330/#:~:text=A%20Cre-dependent%2C%20anterograde%20transsynaptic%20viral%20tracer%20for%20mapping,transsynaptically%2C%20are%20powerful%20tools%20for%20mapping%20neural%20pathways.
AAV-Mediated Anterograde Transsynaptic Tagging: …
https://pubmed.ncbi.nlm.nih.gov/27989459/
Here, we discovered that adeno-associated viruses (AAV1 and AAV9) exhibit anterograde transsynaptic spread properties. AAV1-Cre from transduced presynaptic neurons effectively and specifically drives Cre-dependent transgene expression in selected postsynaptic neuronal targets, thus allowing axonal tracing and functional manipulations of the latter input-defined neuronal …
Anterograde or Retrograde Transsynaptic Circuit Tracing …
https://pubmed.ncbi.nlm.nih.gov/26729030/
Moreover, rVSV enabled transsynaptic tracing of neural circuitry in predictable directions dictated by the viral envelope glycoprotein (G), derived from either VSV or rabies virus (RABV). Anterograde and retrograde labeling, from initial infection and/or viral replication and transmission, was observed in Old and New World monkeys, seahorses, jellyfish, zebrafish, …
Anterograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Anterograde_tracing
Anterograde or Retrograde Transsynaptic Circuit Tracing …
https://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC4776322/
Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use has been ...
A method for anterograde trans-synaptic tracing | Brain Initiative
https://braininitiative.nih.gov/funded-awards/method-anterograde-trans-synaptic-tracing
Arnold, Donald B. To understand how the activation of individual neurons in the brain leads to particular behaviors, it is necessary to identify synaptic connections to downstream neurons. Although considerable information about neuronal circuits has been generated using rabies virus to trace trans-synaptic connections in the retrograde direction, there is no comparable …
Anterograde or retrograde transsynaptic labeling of CNS …
https://pubmed.ncbi.nlm.nih.gov/21825165/
Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors Abstract To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo.
A Cre-dependent, anterograde transsynaptic viral tracer …
https://pubmed.ncbi.nlm.nih.gov/22196330/
A Cre-dependent, anterograde transsynaptic viral tracer for mapping output pathways of genetically marked neurons Neurotropic viruses that conditionally infect or replicate in molecularly defined neuronal subpopulations, and then spread transsynaptically, are powerful tools for mapping neural pathways.
AAV-mediated Anterograde Transsynaptic Tagging: …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5538794/
Advantages of anterograde transsynaptic mapping of functional circuits (A) A brain region “X” is known to mediate a behavior/function of interest. Neurons in X project to multiple target nuclei (“Y”). To map the relevant downstream circuit, conventional method relies on activation of ChR2-expressing X axon terminals in a given target nucleus.
A CRE-DEPENDENT, ANTEROGRADE TRANS-SYNAPTIC …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3275419/
Genetically targetable retrograde trans-synaptic tracer viruses are available to map the inputs to specific neuronal subpopulations, but an analogous tool for mapping synaptic outputs is not yet available. Here we describe a Cre recombinase-dependent, anterograde trans-neuronal tracer, based on the H129 strain of herpes simplex virus (HSV).
Mapping sensory circuits by anterograde transsynaptic …
https://pubmed.ncbi.nlm.nih.gov/24486087/
In this study, we demonstrate that rabies virus can infect sensory neurons in the somatosensory system, is subject to anterograde transsynaptic transfer from primary sensory to spinal target neurons, and can delineate output connectivity with third-order neurons. Anterograde transsynaptic transfer is a feature shared by other classes of primary sensory neurons, …
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