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Lineage tracing meets single-cell omics: opportunities …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307462/
A lineage-tracing experiment that involves marking an individual cell, followed by state analysis of that founder cell’s clonal descendants. Retrospective lineage tracing A lineage-tracing experiment based on phylogenetic reconstruction of endogenous genetic polymorphisms (that is, no experimental intervention).
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
The Theory and Practice of Lineage Tracing Abstract. Lineage tracing is a method that delineates all progeny produced by a single cell or a group of cells. Introduction. In a lineage-tracing experiment, the cells of interest are marked at one timepoint, and the progeny... Nonselective Markers. Many ...
Lineage tracing - PubMed
https://pubmed.ncbi.nlm.nih.gov/22265400/
Abstract. Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 (th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, …
Lineage Tracing: Cell
https://www.cell.com/abstract/S0092-8674%2812%2900003-7
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 th century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and …
Lineage Tracing: Cell
https://www.cell.com/cell/fulltext/S0092-8674(12)00003-7
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 th century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, especially …
Lineage tracing: technology tool for exploring the ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Abstract. Lineage tracing is the most widely used technique to track the migration, proliferation, and differentiation of specific cells in vivo. The currently available gene-targeting technologies have been developing for decades to study organogenesis, tissue injury repairing, and tumor progression by tracing the fates of individual cells.
A Quantitative Lineage-Tracing Approach to Understand ...
https://pubmed.ncbi.nlm.nih.gov/33340352/
Lineage-tracing experiments aim to identify and track the progeny and/or fate of cells. The use of inducible recombinases and fluorescent reporters has been instrumental in defining cellular hierarchies and allowing for the identification of stem cells in an unperturbed in vivo setting. The refinement of these approaches, labeling single cells, and the subsequent quantitative analysis …
Cell Lineage Tracing: Colocalization of Cell Lineage ...
https://experiments.springernature.com/articles/10.1007/978-1-0716-1028-2_18
Cell Lineage Tracing: Colocalization of Cell Lineage Markers with a Fluorescent Reporter. Cell lineage tracing, an old technique which originated in the nineteenth century, regains popularity and relevance due to introduction of a more sensitive tomato fluorescent protein under the control of a ubiquitous promoter (Rosa 26 gene). In. Cell lineage tracing, an old technique …
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Lineage tracing is a technique used to identify and track cell populations in vivo. Genetic lineage tracing is generally performed by combining an inducible Cre expressed in a cell type of interest with a Cre-activated reporter usually expressing a fluorescent protein. This allows indefinite labeling of cells with a visual marker following induction of Cre activity.
How to Track Cell Lineages As They Develop | The Scientist ...
https://www.the-scientist.com/lab-tools/how-to-track-cell-lineages-as-they-develop-32448
Understanding how organisms grow from one cell type to many different cell types is the overall goal of lineage tracing or fate mapping experiments, the first of which date to the late 19th century. Historically, cell lineage tracing studies have taken large pools of cells from mature tissues and inferred their origins retrospectively by staining the cells with...
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