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Retrograde neuronal tracing with cholera toxin B subunit
https://pubmed.ncbi.nlm.nih.gov/7868356/
In this report a comparison is made of three different visualization methods of rat cervical motoneurons retrogradely labelled with cholera toxin B subunit (CTb). CTb conjugates such as CTb-HRP and CTb-FITC or CTb-TRITC, which can be visualized after histochemical detection and by fluorescence microscopy, respectively.
Cholera Toxin Subunit B (CTB) Retrograde tracing from the mouse …
https://www.protocols.io/view/cholera-toxin-subunit-b-ctb-retrograde-tracing-fro-b8ysrxwe
Abstract. This protocol is used to label the neurons and their central terminals of the sensory neurons innervating the colon and bladder in an experimental adult male or female mouse. The protocol is performed under anesthesia and should incorporate all local requirements for standards of animal experimentation, including methods of anesthesia ...
Cholera Toxin B Subunit Shows Transneuronal Tracing after …
https://pubmed.ncbi.nlm.nih.gov/26640949/
Cholera toxin B subunit (CTB) has been extensively used in the past for monosynaptic mapping. For decades, it was thought to lack the ability of transneuronal tracing. In order to investigate whether biotin conjugates of CTB (b-CTB) would pass through transneurons in the rat spinal cord, it was injected into the crushed left sciatic nerve.
Multiple neuroanatomical tract-tracing using fluorescent Alexa …
https://pubmed.ncbi.nlm.nih.gov/19617887/
Cholera toxin subunit B (CTB) is a highly sensitive retrograde neuroanatomical tracer. With the new availability of fluorescent Alexa Fluor (AF) conjugates of CTB, multiple neuroanatomical connections can be reliably studied and compared in the same animal. Here we provide a protocol that describes …. Multiple neuroanatomical tract-tracing using fluorescent Alexa Fluor …
Cholera Toxin B Subunit Shows Transneuronal Tracing after …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4671609/
The cholera toxin B subunit (CTB) binds to cellular surfaces via its receptor monosialoanglioside (GM1) . As a conventional bacterial toxin for neuronal tracing, CTB enters the axons or dendrites by active uptake via nerve terminals, or through severed axons .
Did you choose appropriate tracer for retrograde tracing of retinal ...
https://pubmed.ncbi.nlm.nih.gov/30289890/
Cholera toxin subunit B (CTB) and Fluorogold(FG) are two widely utilized retrograde tracers to assess the number and function of retinal ganglion cells (RGCs). However, the relative advantages and disadvantages of these tracers remain unclear, which may lead to …
The efficacy of the fluorescent conjugates of cholera toxin …
https://pubmed.ncbi.nlm.nih.gov/19621243/
Cholera toxin subunit B (CTB) is a sensitive neuroanatomical tracer that generally transports retrogradely in the nervous system, and has been used extensively in brightfield microscopy. Recently, Alexa Fluor (AF) conjugates of CTB have been made available, which now allows multiple tracing with CTB. In this study, we examined the efficacy of these new AF-CTB …
Did you choose appropriate tracer for retrograde tracing of ... - PLOS
https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0205133
Cholera toxin subunit B (CTB) and Fluorogold(FG) are retrograde tracers widely used in many laboratories [27–33]. CTB is a nontoxic subunit of the cholera toxin protein complex secreted by Vibrio cholerae that binds to cellular surfaces via the pentasaccharide chain of monosialotetrahexosylganglioside .
A light microscope-based double retrograde tracer strategy to …
https://pubmed.ncbi.nlm.nih.gov/17703197/
This protocol describes a double retrograde tracing method to chart divergent projections in the CNS using light microscope techniques. It is based on immunohistochemical visualization of retrograde transport of cholera toxin b-subunit (CTb) and silver enhancement of a …
Anterograde axonal tracing with the subunit B of cholera toxin: a ...
https://pubmed.ncbi.nlm.nih.gov/8815303/
Abstract. We report an improved immunohistochemical protocol for revealing anterograde axonal transport of the subunit B of cholera toxin (CTB) which stains axons and terminals in great detail, so that single axons can be followed over long distances and their arbors reconstructed in their entirety. Our modifications enhance the quality of staining mainly by increasing the penetration …
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