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Identifying Adult Stem Cells Using Cre-Mediated Lineage ...
https://pubmed.ncbi.nlm.nih.gov/26840226/
Lineage-tracing has been used for decades to establish cell fate maps during development. Recently, with the advent of genetic lineage-tracing techniques (employing Cre-lox recombination), it has been possible to permanently mark progenitor/stem cell populations within somatic tissues. In addition, …
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
If lineage tracing is performed using GeneX-Cre, the cell origin of C cells cannot be determined unequivocally. On the other hand, if GeneX-CreER is used, one can selectively label only B cells (if Tamoxifen is applied at timepoint1) or only A cells (if low dosage Tamoxifen is applied at timepoint2 since there are much more A cells than B cells ...
Application of Cre-loxP recombination for lineage tracing ...
https://pubmed.ncbi.nlm.nih.gov/22688702/
The Cre-loxP recombination system is widely used as a genetic tool to achieve conditional gene expression and for lineage tracing. Though extensively used in mice, this technology has only recently been applied to zebrafish. Here we describe Cre-loxP methodology for conditional expression of transgenes in zebrafish and their use in lineage ...
Genetic lineage tracing with multiple DNA recombinases: …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7212637/
For genetic lineage tracing, inducible Cre recombinase was developed with an attempt to achieve temporal and spatial control of recombination. To facilitate inducible recombination, the human estrogen receptor (ER) is fused to Cre recombinase. Because it binds to heat shock proteins (HSPs), the Cre-ER fusion protein is held in the cytoplasm.
Lineage Tracing Using Cux2-Cre and Cux2-CreERT2 Mice
https://pubmed.ncbi.nlm.nih.gov/25996136/
In Cux2-Cre and Cux2-CreERT2 mice that recapitulate the endogenous Cux2 expression pattern, the vast majority of fate-mapped neurons express Satb2 but not Ctip2, confirming that a restricted subset of all neocortical projection neurons belongs to the Cux2 lineage. This Matters Arising paper is in response to Guo et al. (2013), published in Neuron.
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Abstract. Lineage tracing is a technique used to identify and track cell populations in vivo. Genetic lineage tracing is generally performed by combining an inducible Cre expressed in a cell type of interest with a Cre-activated reporter usually expressing a fluorescent protein. This allows indefinite labeling of cells with a visual marker ...
A Method for Lineage Tracing of Corneal Cells Using …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4694035/
Lineage tracing experiments define the origin, fate and behavior of cells in a specific tissue or organism. ... (Cre ERT /Cre ERT) to obtain hemizygous offsprings (Br2.1/+;Cre/+)that are ready for mono-allelic lineage tracing as they all contain a single Br2.1 allele and a single Cre allele. Note: To increase the efficiency of labeling, one ...
A novel lineage-tracing mouse model for studying early ...
https://elifesciences.org/articles/72638
As a control for our lineage-tracing model, we use CAG-Cre plasmid which drives the expression of a self-deleting Cre recombinase, just as in the MmuPV1-lox-Cre-lox plasmid, but it does not encode any MmuPV1 genes. As performed above, pCAG-Cre was delivered to R26R-lox-STOP-lox-eYFP mice after UV-B irradiation and tail skin scarification.
Lineage tracing: technology tool for exploring the ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Lineage tracing is the most widely used technique to track the migration, proliferation, and differentiation of specific cells in vivo. The currently available gene-targeting technologies have been developing for decades to study organogenesis, tissue injury repairing, and tumor progression by tracing the fates of individual cells. Recently, lineage tracing has …
Cre-Lox recombination - Wikipedia
https://en.wikipedia.org/wiki/Cre-Lox_recombination
Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, ... These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally. The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system. ...
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