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Identifying Adult Stem Cells Using Cre-Mediated Lineage ...
https://pubmed.ncbi.nlm.nih.gov/26840226/
Identifying Adult Stem Cells Using Cre-Mediated Lineage Tracing. Lineage-tracing has been used for decades to establish cell fate maps during development. Recently, with the advent of genetic lineage-tracing techniques (employing Cre-lox recombination), it has been possible to permanently mark progenitor/stem cell populations within somatic tissues.
Cre-mediated recombination in cell lineages that express ...
https://pubmed.ncbi.nlm.nih.gov/15682389/
Initial characterization of the PR-Cre mouse underscores the utility of this model to precisely ablate floxed target genes specifically in cell lineages that express the PR. In the wider context of female reproductive tissue ontology, this model will be indispensable in tracing the developmental fate of cell lineages that descend from PR positive progenitors.
Lineage Tracing Mediated by Cre-Recombinase Activity
https://www.jidonline.org/article/S0022-202X(15)37063-9/pdf
In cells in the bulge cell lineage (green cells), the Cre-recombinase mediates the deletion of the loxP site–flanked stop codon in front of the reporter gene and thereby induces reporter gene expression. This permanent genetic modification is passed on to the cell progeny, independent of Cre expression, and facilitates tracing of the cell lineage.
Rosa26-targeted swine models for stable gene over ...
https://pubmed.ncbi.nlm.nih.gov/24503648/
Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing. Rosa26-targeted swine models for stable gene over-expression and Cre-mediated lineage tracing Cell Res. 2014 Apr;24(4):501-4. doi: 10.1038/cr.2014.15. Epub 2014 Feb …
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
If lineage tracing is performed using GeneX-Cre, the cell origin of C cells cannot be determined unequivocally. On the other hand, if GeneX-CreER is used, one can selectively label only B cells (if Tamoxifen is applied at timepoint1) or only A cells (if low dosage Tamoxifen is applied at timepoint2 since there are much more A cells than B cells), thereby determining the exact cell …
Enhancing the precision of genetic lineage tracing using ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6913096/
Because Cre-loxP recombination is sensitive, it can be technically challenging to fully exclude low level or infrequent Cre expression in non-targeted cells, creating a major hurdle for rigorous interpretation of the results of Cre-loxP-mediated genetic lineage tracing 10. Here we present a new system that uses dual recombinases (Cre and Dre) and new reporter lines to resolve the …
Dual Cre and Dre recombinases mediate synchronized …
https://www.sciencedirect.com/science/article/pii/S0021925822004057
Cre-loxP system is a powerful tool for genetic lineage tracing and cell ablation studies . However, the degree of recombination in cells of Rosa26-reporter or Rosa26-iDTR mice varies significantly between different Rosa26 alleles used as readouts ( 31 , 33 ).
Genetic lineage tracing with multiple DNA recombinases: …
https://pubmed.ncbi.nlm.nih.gov/32213599/
Site-specific recombinases, such as Cre, are a widely used tool for genetic lineage tracing in the fields of developmental biology, neural science, stem cell biology, and regenerative medicine. However, nonspecific cell labeling by some genetic Cre tools remains a technical limitation of this recombination system, which has resulted in data misinterpretation and led to many …
Lineage tracing: technology tool for exploring the ...
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Cre-ERT2 mice have also been used in lineage tracing and express a fusion protein of an estrogen receptor (ER) ligand binding region mutant (ERT). Expression or deletion of mutation sites depend on regulation by tamoxifen, whose metabolite 4-OHT (estrogen analog) binds to ERT, inducing Cre-ERT2 enter the nucleus to activate Cre recombinase [ 35 ].
Enhancing the precision of genetic lineage tracing using ...
https://www.nature.com/articles/nm.4437/
The Cre– loxP recombination system is the most widely used technology for in vivo tracing of stem or progenitor cell lineages. The precision of this genetic system largely depends on the ...
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