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Neuronal tracing with DiI: decalcification, cryosectioning, …
https://pubmed.ncbi.nlm.nih.gov/2185313/
We describe three modifications of this tracing method. First, while DiL diffuses along neuronal membranes the tissue can be decalcified in EDTA at 37 degrees C. Tracing in decalcified tissue extends the possible application of the DiI technique to the investigation of neuronal tissue enclosed in bony structures.
Neural tract tracing using Di-I: a review and a new method …
https://pubmed.ncbi.nlm.nih.gov/11074091/
The use of Di-I in tract-tracing is briefly reviewed and a novel delayed-fixation approach to neural tract-tracing in the postmortem human adult brain is reported. Using the new approach, fast Di-I, a highly lipophilic fluorescent dye was injected into a particular region or nucleus and labelled tracts were followed for distances of some 20-40 mm.
DiI tracing in combination with immunocytochemistry for …
https://pubmed.ncbi.nlm.nih.gov/1383644/
It appears that DiI tracing of neural connections in the teleost CNS yields very consistent results and that the combination with immunocytochemistry is very reliable. We present four different basic protocols for combined DiI tracing and immunocytochemistry, with notes on …
Cochlear neuronal tracing for frequency mapping with DiI, …
https://pubmed.ncbi.nlm.nih.gov/18340556/
Cochlear neuronal tracing for frequency mapping with DiI, NeuroVue, and Golgi methods Abstract Conclusions: Labeling experiments using NeuroVue Red dye allowed us to demonstrate individual afferent fiber tracks in the cochlea from the synaptic region of the inner hair cell in the organ of Corti (OC) to the spiral ganglion in Rosenthal's canal.
Neuronal Tracing withDi!:Decalcification, Cryosectioning ...
https://depts.washington.edu/rubelab/personnel/von%20Bartheld1990.pdf
Neuronal Tracing withDi!:Decalcification, Cryosectioning, andPhotoconversion forLight andElectron Microscopic Analysis’ CHRISTOPHER S.VONBARTHELD, DALEE.CUNNINGHAM, andEDWIN W.RUBEL2 Hearing Development Laboratories, University ofWashington Medical School, Seattle, Washington 98195.
Tracing neuronal connections in postmortem human …
https://pubmed.ncbi.nlm.nih.gov/1704107/
These findings indicate that DiI is a useful method for investigating intrinsic local circuit connections in normal aldehyde-fixed postmortem human brain and suggests that DiI could be a powerful tool to examine altered neural connectivity in humans with neurological disease. Publication types Research Support, Non-U.S. Gov't
DiI - Wikipedia
https://en.wikipedia.org/wiki/D282
DiI, pronounced like Dye Aye, also known as DiIC18(3), is a fluorescent lipophilic cationic indocarbocyaninedyewhich is usually made as a perchlorate salt. It is used for scientific staining purposes such as single molecule imaging, fate mapping, electrode marking and neuronal tracing (as DiI is retained in the lipid bilayers).
Cochlear neuronal tracing for frequency mapping with DiI, …
https://utsouthwestern.pure.elsevier.com/en/publications/cochlear-neuronal-tracing-for-frequency-mapping-with-dii-neurovue
In DiI staining, spacial resolution and the number of neurons to be stained are too low to utilize this method to create a characteristic frequency map of the cochlea. The Golgi method mainly stained efferent nerve fibers, resulting in less information on cochlear nerve distribution.
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