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Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
The technique retrograde tracing explores the neuronal retrograde axonal transport and is commonly used to elucidate the relationship between neuronal structure and function across the nervous system. Table 1 lists human genes involved in retrograde axonal transport. ... FG, and DiI have similar labeling efficacies in the retrograde labeling of ...
Example of retrograde tracing observed in DRG after DiI ...
https://researchgate.net/figure/Example-of-retrograde-tracing-observed-in-DRG-after-DiI-injection-into-the-uterus-in_fig1_264311960
Retrograde tracing studies have shown a dense innervation of spinal afferents to the uterus (Berkley et al., 1988; Herweijer et al., 2014). In the mouse, two distinct peaks of …
Retrograde Tracing Technique | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/978-3-540-29805-2_3825
Definition. Retrograde tracing (retrograde labeling) is a neuroanatomical method used to determine the location of the cells of origin of a nervous system pathway. A tracer substance that will be taken up by synaptic terminals (and sometimes by axons) is injected into a region of interest, such as a central nervous system nucleus.
Retrograde tracing and toe spreading after experimental ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473253/
Retrograde tracing with DiI was used to evaluate structural aspects of sciatic nerve regeneration. Representative images and quantification of positively labelled neurons in the spinal cord and DRGs are shown in Figures 2 and and3. 3. Longitudinal sections of the ventral columns revealed positively labelled perikarya in the spinal cord ...
Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp00282.pdf
DiI labeling does not appreciably affect cell viabilit y, deve l op ment, or basic ... In addition to neuronal tracing, lipophilic carbocyanines have many other applications in- ... an alternative to direct application of crystalline dye for retrograde and anterograde neuronal tracing.1,13 A 2.5 mg/mL (0.25% w/v) solution of dye in DMF is ...
DiI tracing of the hypothalamic projection systems during ...
https://pubmed.ncbi.nlm.nih.gov/25538571/
DiI tracing of the hypothalamic projection systems during perinatal development Front Neuroanat. 2014 Dec 4;8:144. doi: 10.3389 ... (IL) and median eminence, mammillary body tracts and reciprocal septohypothalamic connections have been described with retrograde tracing. The available data demonstrate that hypothalamic connections develop with a ...
Anterograde tracing method using DiI to label vagal ...
https://pubmed.ncbi.nlm.nih.gov/17418900/
Therefore, a method using 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) was developed for labe … Anterograde tracing method using DiI to label vagal innervation of the embryonic and early postnatal mouse gastrointestinal tract J Neurosci Methods. 2007 Jul 30;163(2) :213-25. doi ...
Tracers for Membrane Labeling—Section 14.4 | Thermo …
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/tracers-for-membrane-labeling.html
Long-Term Cell Tracing with Membrane Tracers. Lipophilic carbocyanine tracers like DiI are also ideal labels for long-term cell tracing in vivo and in vitro, including studies of cell migration, transplantation, adhesion and fusion. The presence of DiI and DiO in the cell membrane does not appreciably affect cell viability, development or other ...
Frontiers | A Student’s Guide to Neural Circuit Tracing ...
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Anterograde and retrograde labeling with static and trans-synaptic tracers. Tracers are categorized as anterograde or retrograde based on their direction of travel within neurons. Anterograde tracers (green) are taken up by neuronal cell bodies at the injection site and travel down the axon to terminal processes.
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