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Neural tract tracing using Di-I: a review and a new method ...
https://pubmed.ncbi.nlm.nih.gov/11074091/#:~:text=The%20use%20of%20Di-I%20in%20tract-tracing%20is%20briefly,were%20followed%20for%20distances%20of%20some%2020-40%20mm.
Neural tract tracing using Di-I: a review and a new method ...
https://pubmed.ncbi.nlm.nih.gov/11074091/
The use of Di-I in tract-tracing is briefly reviewed and a novel delayed-fixation approach to neural tract-tracing in the postmortem human adult brain is reported. Using the new approach, fast Di-I, a highly lipophilic fluorescent dye was injected into a particular region or nucleus and labelled tracts were followed for distances of some 20-40 mm.
Neuronal tracing with DiI: decalcification, cryosectioning ...
https://pubmed.ncbi.nlm.nih.gov/2185313/
Neuronal tracing with DiI: decalcification, cryosectioning, and photoconversion for light and electron microscopic analysis. The molecule 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is a fluorescent dye which diffuses within cell membranes. The properties of DiI diffusion and fluorescence are maintained in aldehyde-fixed tissue, thereby allowing selective …
Anterograde tracing method using DiI to label vagal ...
https://www.sciencedirect.com/science/article/pii/S0165027007001070
The key modifications included: (1) use of DiI crystals for tracer application to vagal nerve trunks and branches was more effective than DiI solution dried onto glass micropipette tips, (2) addition of 0.1% EDTA to the fixative improved the stability of DiI labeling of nerve elements, especially with long incubation durations and (3) DiI label in vagal fibers and endings appeared …
From DiI to Modern Methods | The Scientist Magazine®
https://www.the-scientist.com/the-marketplace/chemyx-tracing-fibers---from-dii-to-modern-methods-30849
Lipophilic fluorescent neuro-tracers such as DiI (1,1'-Dioctadecyl-3,3,3',3'-Tetramethylindocarbocyanine Perchlorate) allow for anterograde and retrograde axoplasmic flow monitoring. 2 Other retrograde neuro-tracers used to study innervation, and neural cross talk includes Fast Blue and Wheat Germ Agglutinin Horseradish Peroxidase (WGA-HRP). Once …
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