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Fluorescent double-labeling with carbocyanine neuronal tracing an…
https://www.sciencedirect.com/science/article/pii/S0165027008003932#:~:text=The%20fluorescent%20carbocyanine%20dye%20DiI%20%281%2C1%E2%80%B2-dioctadecyl-3%2C3%2C3%E2%80%B2%2C3%E2%80%B2-tetramethylindocarbocyanine%20perchlorate%29%20has,desirable%20to%20combine%20DiI%20labeling%20with%20immunofluorescent%20staining.
DiI tracing in combination with immunocytochemistry for …
https://pubmed.ncbi.nlm.nih.gov/1383644/
It appears that DiI tracing of neural connections in the teleost CNS yields very consistent results and that the combination with immunocytochemistry is very reliable. We present four different basic protocols for combined DiI tracing and immunocytochemistry, with notes on their specific applicability. Owing to their reliability, the protocols ...
Neuronal tracing with DiI: decalcification, cryosectioning, …
https://pubmed.ncbi.nlm.nih.gov/2185313/
The molecule 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (DiI) is a fluorescent dye which diffuses within cell membranes. The properties of DiI diffusion and fluorescence are maintained in aldehyde-fixed tissue, thereby allowing selective neuronal tracing post mortem. We describe three modifications of this tracing method.
DiI/DiA axonal tracing - UConn Health
https://health.uconn.edu/li-lab/wp-content/uploads/sites/129/2017/06/dia_labeling.pdf
DiI/DiA axonal tracing 1. Brains are dissected in PBS, and post-fixed with immersion in 4% PFA at least overnight. (Labeling can be done after one week in PFA) 2. Determine the dye placement location under a dissecting scope. You can first take a digital image of the brain and superimpose a pre-constructed grid on the image to determine the ...
Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp00282.pdf
Sulfonated derivatives of DiI and DiO, and CM-DiI (a thiol-reactive DiI derivative) pro-duce staining that persists after fixation and permeabilization treatments. These tracers are described in instruction manual MP 06999. In addition to neuronal tracing, lipophilic carbocyanines have many other applications in-cluding:
DiI - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/dii
DiI-C 12 is a fluorescent dye which labels the liquid-disordered phase of a lipid membrane and is excited maximally by 549 ... DiI and DiO allow one to trace the retinotectal projections (Baier et al., 1996). When applied to the optic tectum or the optic tract, they can be used to determine the position of ganglion cell perikarya, ...
Tracers for Membrane Labeling—Section 14.4 | Thermo …
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/tracers-for-membrane-labeling.html
Neuronal Tracing Studies with DiI and DiO Analogs. DiI, DiO and DiD are widely used to label neuronal projections in live and fixed tissue (Figure 14.4.4, , ). The dyes insert into the outer leaflet of the plasma membrane and diffuse laterally, producing detailed labeling of fine neuronal projections. DiI has been reported to diffuse about 6 mm ...
Dil Stain (1,1'-Dioctadecyl-3,3,3',3' …
https://www.thermofisher.com/order/catalog/product/D282
DiI is a lipophilic membrane stain that diffuses laterally to stain the entire cell. It is weakly fluorescent until incorporated into membranes. This orangered-fluorescent dye, which is spectrally similar to tetramethylrhodamine, is ... Related applications: Cell Tracing & Tracking.
DII Men's College Outdoor Track & Field - Home | NCAA.com
https://www.ncaa.com/sports/trackfield-outdoor-men/d2
Here's every DII men's outdoor track & field champion since 1963. Grand Valley St. wins 2021 championship.
DiI - Wikipedia
https://en.wikipedia.org/wiki/D282
DiI, pronounced like Dye Aye, also known as DiIC 18 (3), is a fluorescent lipophilic cationic indocarbocyanine dye which is usually made as a perchlorate salt. It is used for scientific staining purposes such as single molecule imaging, fate mapping, electrode marking and neuronal tracing (as DiI is retained in the lipid bilayers).. DiI is manufactured by Invitrogen, which has a series of …
Tracking every DII softball conference tournament for 2022
https://www.ncaa.com/news/softball/article/2022-05-01/tracking-every-dii-softball-conference-tournament-2022
The 2022 DII softball championship tournament is quickly approaching. Track each conference tournament here and see which teams earn an automatic bid to the bracket.
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