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Enhancing Fluorogold-based neural tract tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/S016502701630125X#:~:text=Fluorogold%20%28FG%29%20is%20used%20by%20many%20groups%20to,site%20and%20leads%20to%20motor%20deficits.%20New%20method
Retrograde tracing with Fluoro-Gold: different methods of …
https://pubmed.ncbi.nlm.nih.gov/11074092/
Among the available retrograde fluorescent tracers Fluoro-Gold (FG) is particularly advantageous because it (1) is not only detectable by fluorescence microscopy but also immunocytochemically, resulting in an almost complete staining of the dendritic arbor, (2) is visible in lysosome-like structures allowing for the identification of projection neurons at the ultrastructural level, and …
Fluoro-Gold – Fluorochrome
https://fluorochrome.com/flouro-gold/
Fluoro-Gold is used as a retrograde axonal tracer, although orthograde axonal transport does occur. The survival time should be varied (especially to very short survival times of 12 hours – 2 days) to maximize orthograde transport in the …
Intravitreal fluorogold tracing as a method to label retinal …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343317/
FG is a fluorescent retrograde axonal tracer that is taken up by the axonal terminals (Schmued and Fallon, 1986) and that is widely used to trace retinal ganglion cells (RGCs) from their retinorecipient areas or the optic nerve (Salinas-Navarro et al., 2010; Nadal-Nicolas et al., 2015).
Neuroanatomical tracing of neuronal projections with …
https://pubmed.ncbi.nlm.nih.gov/18369770/
To allow further study of neurons identified and labeled in this way, it is particularly important that tracers are compatible with other tissue processing such as immunocytochemistry. Fluoro-Gold (Fluorochrome Inc., Denver CO) is one such highly flexible fluorescent retrograde marker commonly used for neuronal labeling and neuroanatomical tracing.
Retrograde tracing with Fluoro-Gold: different methods of …
https://www.sciencedirect.com/science/article/pii/S0165027000002922
In studies combining retrograde FG tracing with intracellular injection of Lucifer Yellow, the application of antibodies against Lucifer Yellow may be advantageous to photoconversion. ... Fluorogold as a retrograde tracer used in combination with immunohistochemistry. J. Neurosci. Methods, 29 (1989), pp. 69-72. Article Download PDF …
Enhancing Fluorogold-based neural tract tracing
https://www.sciencedirect.com/science/article/pii/S016502701630125X
The use of the retrograde tracer Fluorogold (FG) is a common technique for determining axonal connections in different experimental paradigms performed in a variety of animal models ranging from lamprey (McClellan et al., 2006) to primate (Giménez-Amaya and Graybiel, 1990, Hayashi et al., 2006). When placed at the level of the axon, FG travels back …
FLUOROGOLD® SLIDE PLATES - GRM Products - (936) …
https://grmcp.com/fluorogold-slide-plates/
Fluorogold® Slide Plates Product Data Sheet (Printable PDF) Benefits: Low Coefficient of Friction Proven Product with over 50 years of service Custom-designed to your application’s requirements Manufactured in America. Design Data: Loading Range: 75 to 3,500psi Temperature Range: …
Does anyone have experience using Fluorogold to trace …
https://www.researchgate.net/post/Does-anyone-have-experience-using-Fluorogold-to-trace-uninjured-peripheral-nerves
I have been trying to trace cells using injection by injecting FluoroGold to their targets with a hamilton syringe for a while now, and for some reason …
How can I minimize the precipitation of fluorogold in tract …
https://www.researchgate.net/post/How-can-I-minimize-the-precipitation-of-fluorogold-in-tract-tracing-experiments
The rabbit anti-Fluorogold from Chemicon (AB153) used to work okay with a fluorescent secondary, but they don't make that anymore. I've tried their replacement (AB153-i), and also Fluorochrome's ...
Did you choose appropriate tracer for retrograde tracing …
https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0205133
Cholera toxin subunit B (CTB) and Fluorogold (FG) are retrograde tracers widely used in many laboratories [ 27 – 33 ]. CTB is a nontoxic subunit of the cholera toxin protein complex secreted by Vibrio cholerae that binds to cellular surfaces via the pentasaccharide chain of monosialotetrahexosylganglioside [ 34 ].
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