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The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/#:~:text=In%20a%20lineage-tracing%20experiment%2C%20the%20cells%20of%20interest,to%20the%20germ-layer%20stage%20%5B%201%2C%202%20%5D.
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
In a lineage-tracing experiment, the cells of interest are marked at one timepoint, and the progeny derived from these marked cells are revealed at a later timepoint. In the 1870s, Charles Otis Whitman observed the early division of leech embryos and followed the fate of individual cells from the one cell stage to the germ-layer stage [1, 2]. This seminal work suggested that a …
Lineage tracing - PubMed
https://pubmed.ncbi.nlm.nih.gov/22265400/
Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 (th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, …
Building a lineage from single cells: genetic techniques …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459401/
When designing a lineage tracing experiment, it is important to consider the strengths and weaknesses of either a prospective or a retrospective approach (FIG. 3). For success in prospective lineage tracing, there must be genetic access to the population in question, whether through a regionally directed method such as viral injection and electroporation, or by using …
Genetic Lineage Tracing of Non-cardiomyocytes in Mice
https://pubmed.ncbi.nlm.nih.gov/32857384/
Genetic lineage tracing is accomplished using bi-transgenic mice, where one allele is altered to express Cre recombinase, and another allele encodes a Cre-dependent genetic reporter protein. Once Cre is activated (constitutive or in response to tamoxifen), the marker gene-expressing cells become indelibly labeled by the reporter protein.
Building a lineage from single cells: genetic techniques …
https://www.nature.com/articles/nrg.2016.159
Lineage relationships are experimentally revealed through fate-mapping methods, and when fate mapping is carried out at single-cell …
Genetic Lineage Tracing of Nonmyocyte Population by …
https://www.ahajournals.org/doi/10.1161/CIRCULATIONAHA.118.034250
Current research on cell fate mapping of endogenous CSCs largely relies on the conventional genetic lineage tracing technology that utilizes the promoters of specific markers expressed by CSCs. However, some proposed stem cell markers are nonspecific to putative CSCs, 12,19 – 21 as they are also expressed by cardiomyocytes. It remains technically challenging to …
Lineage Tracing | Ivory Research
https://www.ivoryresearch.com/samples/lineage-tracing/
In order to trace the lineage of a cell in a non-invasive manner, the genetic recombinase technique has been used to achieve permanent genetic labelling through specific activation of a reporter gene. FLP-FRT (flippase recognition target) recombination system involves the recombination of DNA sequences that lie between the FRT sites by the FLP …
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Lineage tracing with inducible Cre typically involves a pulse–chase experimental design. The “pulse” component allows for identification of cells in which recombination occurs, and it is usually completed by sacrificing animals within 12 h to 2 days after tamoxifen treatment and evaluating labeling histologically.
Trace Your Genetic Ancestry Through National …
https://www.brookings.edu/blog/techtank/2014/04/17/trace-your-genetic-ancestry-through-national-geographics-genographic-project/
Now with the help of National Geographic’s Genographic Project, you can find out. Population geneticist Dr. Spencer Wells and a team at National Geo will send you a …
Unravelling cellular relationships during development and …
https://www.nature.com/articles/s41580-019-0186-3
Tracking the progeny of single cells is necessary for building lineage trees that recapitulate processes such as embryonic development and stem cell differentiation. In classical lineage tracing...
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