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Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816#:~:text=Lineage%20tracing%20is%20a%20technique%20used%20to%20identify,a%20Cre-activated%20reporter%20usually%20expressing%20a%20fluorescent%20protein.
Building a lineage from single cells: genetic techniques …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5459401/
Genetic recombination for lineage tracing. Cell lineage tracing by genetic recombination is able to leverage the expression of recombinase enzymes in a cell-specific or tissue-specific manner to activate the expression of a conditional reporter gene. Two genetically encoded, site-specific recombination systems include Cre-loxP and FLP-FRT.
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Chapter 81 - Methods in lineage tracing Introduction. Lineage tracing is an approach that has been widely used in developmental biology to identify the origin... Cre recombinase. Genetic manipulation has been utilized to direct the specific overexpression or deletion of genes. As... Reporters. ...
Lineage tracing: technology tool for exploring the …
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Lineage tracing is the most widely used technique to track the migration, proliferation, and differentiation of specific cells in vivo. The currently available gene-targeting technologies have been developing for decades to study organogenesis, tissue injury repairing, and tumor progression by tracing the fates of individual cells.
Building a lineage from single cells: genetic techniques …
https://www.nature.com/articles/nrg.2016.159
Genetic lineage tracing of resident stem cells by DeaLT
https://pubmed.ncbi.nlm.nih.gov/30250288/
Genetic lineage tracing based on Cre-loxP recombination provides an effective strategy for inferring cell fate and cell conversion in vivo. However, the determination of t … Unraveling the fates of resident stem cells during tissue regeneration is an important objective in …
Lineage tracing: technology tool for exploring the …
https://pubmed.ncbi.nlm.nih.gov/33059752/
Lineage tracing is the most widely used technique to track the migration, proliferation, and differentiation of specific cells in vivo. The currently available gene-targeting technologies have been developing for decades to study organogenesis, tissue injury repairing, and tumor progression by tracing the fates of individual cells.
Lineage Tracing: Cell
https://www.cell.com/fulltext/S0092-8674(12)00003-7
Figures Figure 1 Different Approaches to Lineage Tracing Figure 2 Lineage Tracing through Genetic Recombination Figure 3 CreER, an Inducible Genetic Recombination System Figure 4 Dual- and Multicolor Reporter Systems Figure 5 Decision Tree for Choosing an Appropriate Lineage Tracing Strategy
Tools and Techniques for Wt1-Based Lineage Tracing
https://pubmed.ncbi.nlm.nih.gov/27417958/
We describe the presently used constitutive or inducible genetic lineage tracing approaches based on the Cre/loxP system utilizing Cre recombinase expression under control of a Wt1 promoter.To make these systems accessible, we provide laboratory protocols that include dissection and processing of the tissues for immunofluorescence and histopathological …
Cellular barcoding: lineage tracing, screening and beyond
https://pubmed.ncbi.nlm.nih.gov/30377352/
Cellular barcoding: lineage tracing, screening and beyond Cellular barcoding is a technique in which individual cells are labeled with unique nucleic acid sequences, termed barcodes, so that they can be tracked through space and time.
In vivo lineage tracing in mice | Nature Reviews Genetics
https://www.nature.com/articles/s41576-018-0048-4
The team built on proof-of-principle concepts of the genome editing of synthetic target arrays for lineage tracing (GESTALT) technique, whereby lineage barcodes that consist of multiple Cas9 target...
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