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High resolution, plasmid-driven cell lineage tracing reveals ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086637/#:~:text=More%20recently%2C%20nuclear%20localized%20fluorescent%20proteins%20%28nXFPs%29%20expressed,embryo%20increases%20during%20development%20%28Zhang%20and%20Weisblat%2C%202005%29.
Improved fluorescent compounds for tracing cell lineage.
https://www.ncbi.nlm.nih.gov/pubmed/2581834
Gimlich RL, Braun J. In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 X 10 (3), and with one or more fluorophore molecules covalently coupled to each dextran chain. These fluorescent dextrans are brighter than commercially obtainable …
Improved fluorescent compounds for tracing cell lineage
https://www.sciencedirect.com/science/article/pii/0012160685904762
Fluorescent dextran and fluorescent dextran-amine are easy to synthesize and represent superior lineage tracers that can be adapted to a variety of experimental designs in embryos of many species. This work was supported by USPHS Grant GM19363 to J. C. Gerhart, and by NIH Grants NS12818 and HD17088, NSF Grant BNS 79-12400, and a grant from the …
Improved fluorescent compounds for tracing cell lineage
https://www.sciencedirect.com/science/article/abs/pii/0012160685904762
Improved fluorescent compounds for tracing cell lineage ... In this note simple methods for the synthesis of several new fluorescent cell lineage tracers are described. These are fluorescent dextrans with average molecular weights of approximately 11 × 10 3 ...
Improved fluorescent compounds for tracing cell lineage.
https://www.semanticscholar.org/paper/Improved-fluorescent-compounds-for-tracing-cell-Gimlich-Braun/efb3aa25fcd73c4890adc17adea053b5fcb6c439
Semantic Scholar extracted view of "Improved fluorescent compounds for tracing cell lineage." by R. Gimlich et al. ... {Improved fluorescent compounds for tracing cell lineage.}, author={Robert L. Gimlich and J Braun}, journal={Developmental biology}, year={1985}, volume={109 2}, pages={ 509-14 } } R. Gimlich, J. Braun; Published 1 June 1985 ...
Improved fluorescent compounds for tracing cell lineage.
https://europepmc.org/article/MED/2581834
A method is also described for covalently attaching free amino groups to fluorescent dextran to make the tracers cofixable with cellular constituents by aldehyde treatment. Fluorescent dextran-amine tracers allow embryonic cell lineages to be studied in fixed, permeabilized, or sectioned embryos.
Cell Lineage Tracing: Colocalization of Cell Lineage …
https://pubmed.ncbi.nlm.nih.gov/33197022/
Cell lineage tracing, an old technique which originated in the nineteenth century, regains popularity and relevance due to introduction of a more sensitive tomato fluorescent protein under the control of a ubiquitous promoter (Rosa 26 gene). In addition, various tissue specific CreERT2 mouse lines a …
High resolution, plasmid-driven cell lineage tracing …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3086637/
More recently, nuclear localized fluorescent proteins (nXFPs) expressed from injected mRNAs have been used for cell lineage analysis in this system (Zhang and Weisblat, 2005). nXFPs are useful because they permit more precise determination of cell position and cell number than can be achieved with tracers distributed throughout the cytoplasm, especially as the cellular …
Tracking cell lineages to improve research reproducibility …
https://www.nature.com/articles/s41587-021-00928-1
S.Z. is a co-founder of FIND Genomics, a company that aims to improve reproducible cell-based science and develops cell lineage tracking software. N.E.S is an advisor to Vertex. Additional information
Scientists Develop New Method to Trace Cell Lineage
https://news.feinberg.northwestern.edu/2020/07/scientists-develop-new-method-to-trace-cell-lineage/
Northwestern Medicine scientists have developed a new method to trace cell lineage, which can help determine the origin of mammalian neuron cell populations from progenitor cells, according to a study published in the journal Developmental Cell. Neural progenitor cells are early descendants of stem cells that can differentiate into thousands of ...
Stem cells: lineage tracing lets single cells talk about …
https://www.nature.com/articles/s41592-018-0016-0
A flurry of new approaches enable lineage tracing at single-cell resolution. ... 16–32 copies of a red fluorescent protein in the zebrafish line Zebrabow-M. ... will deliver improved, more ...
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