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Metabolomics and Isotope Tracing - PubMed
https://pubmed.ncbi.nlm.nih.gov/29727671/
Metabolomics and Isotope Tracing Great strides have been made over the past decade toward comprehensive study of metabolism. Mass spectrometry (MS) has played a central role by enabling measurement of many metabolites simultaneously. Tracking metabolite labeling from stable isotope tracers can in addition reveal pathway activities. …
Isotope Tracing in Natural Systems | ANSTO
https://www.ansto.gov.au/our-science/environment/isotope-tracing-natural-systems
The Isotope Tracing in Natural Systems team can provide customised services to suit the particular requirements of each client. Applications The facilities can be used in a broad range of fields including: Hydrology Ecology and palaeoecology Geomorphology and geochronology Climate and atmospheric science Food provenance Capabilities
Isotope tracing assisted metabolic profiling: Application …
https://pubmed.ncbi.nlm.nih.gov/30567669/
Isotope-tracing facilitates the understanding of metabolic regulation in biological systems. Depending on the selection of tracers, some essential metabolites cannot be traced. A comprehensive understanding of the regulated pathways can only be achieved with focus beyond labeled metabolites.
Metabolomics and isotope tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6034115/
Metabolomics, with or without isotope tracing, involves three basic steps: (i) sample preparation, (ii) metabolome measurement, and (iii) data analysis ( Figure 3 ). While the measurement step involves the glamorous technology, sample preparation and data analysis are equally important. Figure 3 Steps in metabolomic analysis
Isotope tracing reveals glycolysis and oxidative …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8045768/
In vivo, intra-operative isotope tracing with 13 C-labeled nutrients is an informative means to assess some aspects of tumor metabolism, particularly central pathways such as glycolysis and the TCA cycle. To date, this technique has been used to characterize nutrient utilization in several forms of adult cancer. Here we demonstrate that isotope ...
Spatially resolved isotope tracing reveals tissue …
https://www.nature.com/articles/s41592-021-01378-y
Infused isotope tracers can contribute to tissue metabolites either directly (through metabolism locally within tissues or cells) or indirectly (after being converted elsewhere in the body into a...
Spatially resolved isotope tracing reveals tissue …
https://pubmed.ncbi.nlm.nih.gov/35132243/
We couple stable-isotope-labeled nutrient infusion to matrix-assisted laser desorption ionization imaging mass spectrometry (iso-imaging) to quantitate metabolic activity in mammalian tissues in a spatially resolved manner. In the kidney, we visualize gluconeogenic flux and glycolytic flux in the cortex and medulla, respectively.
Isotopic labeling - Wikipedia
https://en.wikipedia.org/wiki/Isotopic_labeling
Isotopic labeling (or isotopic labelling) is a technique used to track the passage of an isotope (an atom with a detectable variation in neutron count) through a reaction, metabolic pathway, or cell. The reactant is 'labeled' by replacing specific atoms by their isotope. The reactant is then allowed to undergo the reaction.
Stable Isotope Tracing Metabolomics to Investigate the …
https://pubmed.ncbi.nlm.nih.gov/32756373/
However, to render their use as efficient as possible, the mechanism of action of these phytochemicals needs to be well characterized. Stable isotope tracing is an essential technology to study the molecular mechanisms by which nutraceuticals modulate …
USGS -- Isotope Tracers -- Resources
https://wwwrcamnl.wr.usgs.gov/isoig/period/o_iig.html
USGS -- Isotope Tracers -- Resources Resources on Isotopes Periodic Table--Oxygen Oxygen has three stable isotopes: 16 O (99.63%), 17 O (0.0375%) and 18 O (0.1995). Ratios of 18 O to 16 O in waters, rocks, and most solutes are reported in ‰ (permil) relative to Vienna Standard Mean Ocean Water (VSMOW).
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