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Kymograph - Wikipedia
https://en.wikipedia.org/wiki/Kymograph
Kymograph - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/medicine-and-dentistry/kymograph
The x and y axes of the generated kymograph correspond to the distance along the microtubule and time points, respectively. In the kymograph, the movement of a Qdot-labeled dynein along the microtubule appears as a sloped trajectory (Fig. 4). Motility parameters such as velocity, run length, duration time on a microtubule, and directionality of the movement can be obtained by …
Kymograph Instruction Manual - Harvard Apparatus
https://www.harvardapparatus.com/media/manuals/Product%20Manuals/Kymograph%20Instruction%20Manual.pdf
pressure applied to effect a good trace. A Kymograph speed of 2.5mm/sec is recommended. If it is desired to remove the cylinder or replace a chart during a series of experiments, it is advisable to unplug the writing lever from the fork fitting, rather than move the bath as a whole. This will
Video Spot Tracker Tutorial: Kymograph | CISMM
https://cismm.web.unc.edu/resources/tutorials/video-spot-tracker-tutorial-kymograph/
Set the tracking kernel to a 10-pixel radius cone and turn off the dark_spot control. Select the kymograph control, which will cause two new windows to appear. Place four trackers; the first two on the spindle ends and the next two on the posteroir and anterior ends of the cell (I do not know if these are reversed in the image below):
KymoButler, a deep learning software for automated …
https://pubmed.ncbi.nlm.nih.gov/31405451/
Kymographs are graphical representations of spatial position over time, which are often used in biology to visualise the motion of fluorescent particles, molecules, vesicles, or organelles moving along a predictable path.
A Kymograph and Its Use
https://scholarworks.uni.edu/cgi/viewcontent.cgi?article=7320&context=pias
Instead of a kymograph or myograph of foreign manufacture, a revolving upright cylinder may be constructed for taking a 2 Proceedings of the Iowa Academy of Science, Vol. 2 [1894], No. 1, Art. 17 ... the tracing thus preserved be oiled, it becomes thin enough to . be . used for printing blue print copies of it. VOLATILITY OF MERCURIC CHLORIDE.
KymoButler: A deep learning software for automated …
https://www.biorxiv.org/content/10.1101/405183v1.full.pdf
In these kymographs, individual EB1- GFP trajectories are visually distinguishable as bright lines. We traced these trajectories by hand and colour- available under aCC-BY-NC-ND 4.0 International license . not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
Simple and Direct Assembly of Kymographs from Movies …
https://onlinelibrary.wiley.com/doi/full/10.1111/tra.12127
In a kymograph, the movement of an individual cargo is shown as a trajectory (i.e. a locus). If there are multiple cargos, their movements are shown as multiple trajectories, which often intersect or overlap with each other. The most important attribute of a kymograph is that the movement of a target cargo can be analyzed by tracing its trajectory.
GitHub - mrsata/Microtubule_Kymograph_Analysis
https://github.com/mrsata/Microtubule_Kymograph_Analysis
Use the segmented line (polyline) tool to trace microtubule activities on the kymographs. Start at the root, click at each point where the microtubule changes its phases (growth/shrink/pause), and stop when the microtubule shrinks back to the root. Once you are content with the current tracking and statistics, you can press output to save them.
Using a spirometer to investigate human lung function
https://practicalbiology.org/cells-to-systems/ventilation-systems/using-a-spirometer-to-investigate-human-lung-function
If using a kymograph, make sure that the pen makes a mark on the lower part of the drum. ii Let a specific volume of air into the spirometer chamber (say 500 cm 3). Record the position of the chamber by making a mark on the kymograph. iii Do this again, letting in the same volume of air each time, until the chamber has risen as high as possible.
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