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Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816#:~:text=%20Chapter%2081%20-%20Methods%20in%20lineage%20tracing,that%20express%20a%20marker%20gene%20following...%20More%20
Methods in lineage tracing - ScienceDirect
https://www.sciencedirect.com/science/article/pii/B9780128148419000816
Chapter 81 - Methods in lineage tracing Introduction. Lineage tracing is an approach that has been widely used in developmental biology to identify the origin... Cre recombinase. Genetic manipulation has been utilized to direct the specific overexpression or deletion of genes. As... Reporters. ...
The Theory and Practice of Lineage Tracing - PMC
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4618107/
The Theory and Practice of Lineage Tracing Abstract. Lineage tracing is a method that delineates all progeny produced by a single cell or a group of cells. Introduction. In a lineage-tracing experiment, the cells of interest are marked at one timepoint, and the progeny... Nonselective Markers. Many ...
Lineage-tracing methods and the kidney - PubMed
https://pubmed.ncbi.nlm.nih.gov/24088959/
Genetic lineage tracing offers a powerful tool to decipher cellular hierarchies in both development and disease because it allows the progeny of a single cell, or group of cells, to be tracked unambiguously. Recent advances in this field include the use of inducible recombinases, multicolor reporters, and mosaic analysis.
Methods for lineage tracing on the organism-wide level
https://pubmed.ncbi.nlm.nih.gov/29175321/
Furthermore, lineage tracing is a powerful approach for understanding the origin of developmental defects as well as the origin of diseases such as cancer. There is now a variety of complementary approaches for identifying lineage relationships, ranging from direct observation of cell divisions by light microscopy to genetic labeling of cells using inducible recombinases and fluorescent …
Lineage tracing: technology tool for exploring the …
https://stemcellres.biomedcentral.com/articles/10.1186/s13287-020-01941-y
Labeling method. DNA transfection and viral transduction. In principle, DNA transfection and viral transduction mediate the active or passive introduction of foreign ... In situ hybridization. Gene-targeting technology. Barcode technology. Single-cell sequencing methods.
Multicolor lineage tracing methods and intestinal tumors
https://pubmed.ncbi.nlm.nih.gov/23307044/
Multicolor lineage tracing methods and intestinal tumors Abstract The generation of chimeras, which is now a standard technology for producing gene modified mutant mice, was originally developed as a tool for developmental biology. However, the application of conventional single marker chimeric mice for developmental study was initially limited.
Lineage tracing - PubMed
https://pubmed.ncbi.nlm.nih.gov/22265400/
Abstract. Lineage tracing is the identification of all progeny of a single cell. Although its origins date back to developmental biology of invertebrates in the 19 (th) century, lineage tracing is now an essential tool for studying stem cell properties in adult mammalian tissues. Lineage tracing provides a powerful means of understanding tissue development, homeostasis, and disease, …
Lineage tracing meets single-cell omics: opportunities …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7307462/
Lineage tracing meets single-cell omics: opportunities …
https://www.nature.com/articles/s41576-020-0223-2/
Traditionally reliant on microscopy, lineage-tracing approaches have recently evolved to allow the tracking of cell clones via sequencing of inherited DNA sequences, or ‘ …
Lineage Tracing: Cell
https://www.cell.com/abstract/S0092-8674%2812%2900003-7
When lineage tracing by direct observation is not possible, techniques are required to label the cells of interest. In the early 20 th century, “vital dyes,” which label cells without killing them, were used to directly mark cells in amphibian embryos and follow the fate of their progeny during gastrulation ( Vogt, 1929 ) ( Figure 1 B).
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