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Monosynaptic restriction of transsynaptic tracing from single ...
https://pubmed.ncbi.nlm.nih.gov/17329205/#:~:text=Monosynaptic%20restriction%20of%20transsynaptic%20tracing%20from%20single%2C%20genetically,cell%20group%20or%2C%20especially%2C%20to%20a%20single%20cell.
Monosynaptic restriction of transsynaptic tracing from …
https://pubmed.ncbi.nlm.nih.gov/17329205/
Abstract. There has never been a wholesale way of identifying neurons that are monosynaptically connected either to some other cell group or, especially, to a single cell. The best available tools, transsynaptic tracers, are unable to distinguish weak direct connections from strong indirect ones. Furthermore, no tracer has proven potent enough to label any connected neurons …
Monosynaptic Restriction of Transsynaptic Tracing from …
https://www.sciencedirect.com/science/article/pii/S0896627307000785
The result is an inescapable ambiguity in the number of synapses crossed: transsynaptic tracers are incapable of distinguishing strong indirect connections from weak direct ones. They therefore cannot be used in their current form to determine monosynaptic connections in any kind of comprehensive way.
Monosynaptic Restriction of Transsynaptic Tracing from …
https://www.cell.com/fulltext/S0896-6273(07)00078-5
Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically Targeted Neurons Summary There has never been a wholesale way of identifying neurons that are monosynaptically connected either to some other cell group or, especially, to a single cell.
Monosynaptic Restriction of Transsynaptic Tracing From …
https://www.academia.edu/es/13938537/Monosynaptic_Restriction_of_Transsynaptic_Tracing_From_Single_Genetically_Targeted_Neurons
As stated above, the gene gun typically 644 Neuron 53, 639–647, March 1, 2007 ª2007 Elsevier Inc. Neuron Monosynaptic Restriction of Transsynaptic Tracing transfects large numbers of cells within a slice rather than facilitate studies of the functional properties and interac- the single cell that would be required to ideally test the tions between connections that are presently not …
Monosynaptic restriction of transsynaptic tracing from single ...
https://wickershamlab.org/2007/03/01/monosynaptic-restriction-of-transsynaptic-tracing-from-single-genetically-targeted-neurons/
Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons – Wickersham Lab Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM. Neuron. 2007 Mar 1;53 (5):639-47.
Monosynaptic Restriction of Transsynaptic Tracing from …
https://www.semanticscholar.org/paper/Monosynaptic-Restriction-of-Transsynaptic-Tracing-Wickersham-Lyon/2bda4a60ca5d273fb9092aae3f7db5730fd86caf
DOI: 10.1016/j.neuron.2007.01.033 Corpus ID: 8240184; Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically Targeted Neurons @article{Wickersham2007MonosynapticRO, title={Monosynaptic Restriction of Transsynaptic Tracing from Single, Genetically Targeted Neurons}, author={Ian R. Wickersham and David C. …
Monosynaptic Restriction of Transsynaptic Tracing from …
https://www.researchgate.net/publication/6477791_Monosynaptic_Restriction_of_Transsynaptic_Tracing_from_Single_Genetically_Targeted_Neurons
There has never been a wholesale way of identifying neurons that are monosynaptically connected either to some other cell group or, especially, to a single cell. The best available tools,...
Addgene: Monosynaptic restriction of transsynaptic …
https://www.addgene.org/browse/article/1640/
Monosynaptic restriction of transsynaptic tracing from single, genetically targeted neurons. Wickersham IR, Lyon DC, Barnard RJ, Mori T, Finke S, Conzelmann KK, Young JA, Callaway EM Neuron. 2007 Mar 1. 53 (5):639-47. PubMed Article
Monosynaptic tracing: a step-by-step protocol - PubMed
https://pubmed.ncbi.nlm.nih.gov/31408693/
Abstract. Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons …
Monosynaptic Tracing using Modified Rabies Virus …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4471831/
Overview of Monosynaptic Tracing Methodology (A) Schematic representation of the lentivector constructs used in the study. The tracing vector labels cells with a histone-tagged GFP, the TVA receptor necessary for ΔG-rabies infection as well as the rabies GP that allows ΔG-rabies to transmit across synapses.
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