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Pseudorabies virus tracing of neural pathways between the ...
https://pubmed.ncbi.nlm.nih.gov/10713575/#:~:text=The%20transneuronal%20tracer%2C%20pseudorabies%20virus%20%28PRV%29%2C%20was%20used,analgesia%20and%20abbreviation%20of%20estrus%20by%20vaginocervical%20stimulation.
Pseudorabies virus as a transneuronal tract tracing tool ...
https://www.ncbi.nlm.nih.gov/pubmed/8542383
Pseudorabies virus as a transneuronal tract tracing tool: specificity and applications to the sympathetic nervous system. Strack AM(1). Author information: (1)Dept. of Physiology, Univ. of California, San Francisco 94143-0444, USA.
Pseudorabies virus tracing of neural pathways between …
https://pubmed.ncbi.nlm.nih.gov/10713575/
The transneuronal tracer, pseudorabies virus (PRV), was used to identify pathways from the uterine cervix which may be involved in induction of analgesia and abbreviation of estrus by vaginocervical stimulation. In Experiment I, PRV immunoreactivity (PRV-IR) in brain and spinal cord was examined 3-5 days after injection into the cervix of ovariectomized (OVX) female rats …
[Studies on neuronal tracing with pseudorabies virus]
https://pubmed.ncbi.nlm.nih.gov/25118391/
With its abilities of trans-synaptic tracing and self-replication and wide host range, pseudorabies virus (PRV) has been applied in the field of neuroanatomy since the 1970s. Four decades of PRV application have made many advances in researches on neuronal tracing with PRV. Mechanism studies focused on investigating infection of primary neurons and tracing direction in …
Molecular Biology of Pseudorabies Virus: Impact on ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1197806/
Pseudorabies virus (PRV) is a pathogen of swine resulting in devastating disease and economic losses worldwide. ... Dual tract tracing experiments have employed PRV-Bartha-derived mutants to determine whether different targets in the peripheral nervous system are under control of a common region of the central nervous system.
Frontiers | A Student’s Guide to Neural Circuit Tracing ...
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Rabies and pseudorabies virus (PRV, a member of the alphaherpes virus family) were developed as retrograde trans-synaptic tracers in the latter decades of the 20th century ( …
USDA APHIS | Pseudorabies (PRV)
https://www.aphis.usda.gov/aphis/ourfocus/animalhealth/nvap/NVAP-Reference-Guide/Control-and-Eradication/Pseudorabies
Transmission of PRV can occur by direct nose-to-nose;, venereally , including insemination with infected semen or transplacental transmission. Indirect transmission can occur by inhalation of aerosolized virus or ingestion of contaminated water. PRV is still found in feral swine populations.
Viral neuronal tracing - Wikipedia
https://en.wikipedia.org/wiki/Viral_Neuronal_Tracing
A PC12 neuron infected with pseudorabies virus PRV GS443 (labeled in green). The green dots moving away from the cell body demonstrates anterograde transport . Viral neuronal tracing is the use of a virus to trace neural pathways , providing a self-replicating tracer .
Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
The pseudorabies virus (PRV; Bartha strain), for example, may be used as a suitable tracer due to the propensity of the infection to spread upstream through a pathway of synaptically linked neurons, thus revealing the nature of their circuitry.
Transsynaptic Tracing from Peripheral Targets with ...
https://www.jove.com/v/50672/transsynaptic-tracing-from-peripheral-targets-with-pseudorabies-virus
Transsynaptic tracing has become a powerful tool for analyzing central efferents regulating peripheral targets through multi-synaptic circuits. Here we present a protocol that exploits the transsynaptic pseudorabies virus to identify and localize a functional brain circuit, followed by classical tract tracing techniques to validate specific connections in the circuit between …
Viral tracing identifies distributed columnar organization ...
https://www.pnas.org/content/103/33/12592
To probe synaptic connectivity in the OB, we injected a retrograde-specific strain of the pseudorabies virus into the rat OB and piriform cortex. The viral-staining patterns revealed a striking columnar organization that extended across all layers of the OB from the glomeruli to the deep granule cell layer.
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