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Radioactive Amino Acids - Big Chemical Encyclopedia
https://chempedia.info/info/radioactive_amino_acids/#:~:text=Radioactive%20amino%20acids%20are%20commonly%20used%20to%20follow,acid%20acts%20as%20a%20tracer%20without%20causing%20imbalance.
Radioactive Amino Acids - Big Chemical Encyclopedia
https://chempedia.info/info/radioactive_amino_acids/
Radioactive amino acids are commonly used to follow protein synthesis. The procedure is in general similar to that used to follow synthesis of DNA, but in this case the cell s growth medium already contains high concentrations of amino …
Radiolabeled amino acids for oncologic imaging - PubMed
https://pubmed.ncbi.nlm.nih.gov/23708197/
Radiolabeled amino acids (AAs) are an important class of imaging agents for PET and SPECT that target the increased levels of AA transport by many tumor cells. System L AA transport has been a major focus of tracer development, and work in this field has led to several tracers that are effective for imaging brain tumors.
Notes on the application of radioactive amino acids for …
https://www.ncbi.nlm.nih.gov/pubmed/803396
1. Brain Res. 1975 Feb 28;85(2):267-71. Notes on the application of radioactive amino acids for the tracing of neuronal connections. Künzle H.
Radioactive tracer - Wikipedia
https://en.wikipedia.org/wiki/Radioactive_tracer
Radioactive Tracers in Biology - National Institutes of …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2599095/
Radioactive Tracers in Biology. Yale J Biol Med. 1951 Jun; 23 (6): 544–545.
Amino Acid transporter-targeted radiotracers for …
https://pubmed.ncbi.nlm.nih.gov/22664245/
Amino Acid transporter-targeted radiotracers for molecular imaging in oncology Abstract Amino acids are required for sustenance of continuous uncontrolled growth of tumor cells, and upregulation of amino acid transporter expression has often been observed in tumor cells to facilitate their accelerated rates of amino acid uptake.
Labelled Amino Acids
https://www.protocols.io/view/labelled-amino-acids-b8rjrv4n
Basically, isotope-labeled amino acids have identical structures and properties as non-labeled amino acids. The trends of isotopes-labeled amino acids can be observed by analytical methods such as mass spectrometry and nuclear magnetic resonance. Therefore, isotope-labeled amino acids serve as safe, effective, and convenient tracer tools for scientific …
Metabolic Labeling and Chemoselective Ligation
https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/metabolic-labeling-chemoselective-ligation.html
Examples of radioactive tracers and applications include 3H thymidine uptake for cell proliferation assays, 35S methionine labeling for protein synthesis determination, 32P orthophosphate labeling for in vivo kinase assays, and 14C-labeled D-glucose update for determination of cellular metabolism rates. Although radioactive isotopes are easily detected …
Pulse Chase Experiment Flashcards - Quizlet
https://quizlet.com/122645307/pulse-chase-experiment-flash-cards/
The experiment showed that newly synthesized proteins go through the. Radioactive amino acids. What were the tracking pulse-labeled proteins during the chase? Pulse. During what phase of the experiment did was the label added. Chase. During what phase of the experiment was the cell washing out the unincorporated label? Process of movement of ...
methods Flashcards | Quizlet
https://quizlet.com/314568066/methods-flash-cards/
determiningthemultisynapticconnectionsofaregionbyinjectingatracer(e.g., herpes/pseudorabies virus; radioactive amino acids) that is taken up by first order neurons, transported, released at synapses, taken up by second order neurons, etc.; different viral strains are used for either anterograde or retrograde tracing
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