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Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
The technique retrograde tracing explores the neuronal retrograde axonal transport and is commonly used to elucidate the relationship between neuronal structure and function across the nervous system. Table 1 lists human genes involved in …
Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
Neuronal retrograde tracing using the dye DiI (1,1-didodecyl …
http://www.amg-925.com/2018/08/14/neuronal-retrograde-tracing-using-the-dye-dii-11-didodecyl-3333-tetramethylindocarbocyanine-perchlorate-in-conjunction/
Neuronal retrograde tracing using the dye DiI (1,1-didodecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate), in conjunction with immunohistochemical detection of choline acetyltransferase (ChAT) and vasoactive intestinal peptide (VIP), were utilized to recognize the innervation from the mucosa from the guinea-pig proximal colon by submucosal …
Example of retrograde tracing observed in DRG after DiI …
https://researchgate.net/figure/Example-of-retrograde-tracing-observed-in-DRG-after-DiI-injection-into-the-uterus-in_fig1_264311960
Retrograde tracing studies have shown a dense innervation of spinal afferents to the uterus (Berkley et al., 1988; Herweijer et al., 2014). In the mouse, two distinct peaks of …
Retrograde tracing and toe spreading after experimental …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3473253/
In particular, retrograde tracing experiments indicate the type of axons (from motor [spinal cord] versussensory neurons [dorsal root ganglia]) that regenerate. Yet, none of the individual methods cover all the possible therapeutic effects of peripheral nerve injury repair.
Retrograde Tracing Technique | SpringerLink
https://link.springer.com/referenceworkentry/10.1007/978-3-540-29805-2_3825
Definition Retrograde tracing (retrograde labeling) is a neuroanatomical method used to determine the location of the cells of origin of a nervous system pathway. A tracer substance that will be taken up by synaptic terminals (and sometimes by axons) is injected into a region of interest, such as a central nervous system nucleus.
Lipophilic Tracers—Dil, DiO, DiD, DiA, and DiR
https://assets.thermofisher.com/TFS-Assets/LSG/manuals/mp00282.pdf
Pressure microinjection of a small bolus of concentrated dye solution is an alternative to direct application of crystalline dye for retrograde and anterograde neuronal tracing.1,13A 2.5 mg/mL (0.25% w/v) solution of dye in DMF is typically used.
Tracers for Membrane Labeling—Section 14.4 | Thermo …
https://www.thermofisher.com/us/en/home/references/molecular-probes-the-handbook/fluorescent-tracers-of-cell-morphology-and-fluid-flow/tracers-for-membrane-labeling.html
Lipophilic carbocyanine tracers like DiI are also ideal labels for long-term cell tracing in vivo and in vitro, including studies of cell migration, transplantation, adhesion and fusion. The presence of DiI and DiO in the cell membrane does not appreciably affect cell viability, development or other basic physiological properties.
DiI tracing of the hypothalamic projection systems during …
https://pubmed.ncbi.nlm.nih.gov/25538571/
DiI tracing of the hypothalamic projection systems during perinatal development Front Neuroanat. 2014 Dec 4;8:144. doi: 10.3389 ... (IL) and median eminence, mammillary body tracts and reciprocal septohypothalamic connections have been described with retrograde tracing. The available data demonstrate that hypothalamic connections develop with a ...
A Student’s Guide to Neural Circuit Tracing - Frontiers
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Tracers are categorized as anterograde or retrograde based on their direction of travel within neurons. Anterograde tracers (green) are taken up by neuronal cell bodies at the injection site and travel down the axon to terminal processes. Retrograde tracers (blue) are taken up by terminals and travel back to the cell body.
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