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Retrograde tracing with Fluoro-Gold: different methods of …
https://pubmed.ncbi.nlm.nih.gov/11074092/
among the available retrograde fluorescent tracers fluoro-gold (fg) is particularly advantageous because it (1) is not only detectable by fluorescence microscopy but also immunocytochemically, resulting in an almost complete staining of the dendritic arbor, (2) is visible in lysosome-like structures allowing for the identification of projection …
Fluoro-Gold: a new fluorescent retrograde axonal tracer …
https://pubmed.ncbi.nlm.nih.gov/2425899/
A new fluorescent dye, Fluoro-Gold, has been demonstrated to undergo retrograde axonal transport. Its properties include intense fluorescence, extensive filling of dendrites, high resistance to fading, no uptake by intact undamaged fibers of passage, no diffusion from labeled cells, consistent and p …
Fluorogold as a retrograde tracer used in combination …
https://pubmed.ncbi.nlm.nih.gov/2668650/
The fluorescent dye Fluorogold has been recently introduced as a retrograde neuronal tracer that can be used in combination with immunohistochemistry. It has, however, lately been reported that immunohistochemical processes induced rapid photobleaching of Fluorogold.
Fluorogold as a retrograde tracer used in combination …
https://www.sciencedirect.com/science/article/pii/016502708990109X
Fluorogold is a new retrograde tracer which was first introduced by Schmued and Fallon (1986). Along with its many unique properties as a good tracer it was found to be compatible with many neurohistochemical techniques, among which immunohistochemistry.
Did you choose appropriate tracer for retrograde tracing …
https://pubmed.ncbi.nlm.nih.gov/30289890/
Cholera toxin subunit B (CTB) and Fluorogold(FG) are two widely utilized retrograde tracers to assess the number and function of retinal ganglion cells (RGCs). However, the relative advantages and disadvantages of these tracers remain unclear, which may lead to …
Retrograde axonal tracing with fluorescent markers
https://pubmed.ncbi.nlm.nih.gov/18428673/
The growth of fluorescence imaging technology and the development of sensitive fluorescent retrograde tracers has provided many new approaches for analyzing neuronal circuits. Fluorescent markers provide unparalleled opportunity for combining axonal tract tracing with techniques such as immunohistochemistry or physiological recording.
Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
Fluoro-Gold (FG) is a potent water-soluble crystalline retrograde neuronal tracer with following properties: strong fluorescence, extensive filling of neurons, labeling neuronal soma and dendrites, high resistance to bleaching, no uptake by intact undamaged fibers of passage, no diffusion from labeled cells; and not transported transsynaptically.
Did you choose appropriate tracer for retrograde tracing …
https://journals.plos.org/plosone/article?id=10.1371%2Fjournal.pone.0205133
Cholera toxin subunit B (CTB) and Fluorogold (FG) are retrograde tracers widely used in many laboratories [ 27 – 33 ]. CTB is a nontoxic subunit of the cholera toxin protein complex secreted by Vibrio cholerae that binds to cellular surfaces via the pentasaccharide chain of monosialotetrahexosylganglioside [ 34 ].
Intravitreal fluorogold tracing as a method to label retinal …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8343317/
FG is a fluorescent retrograde axonal tracer that is taken up by the axonal terminals (Schmued and Fallon, 1986) and that is widely used to trace retinal ganglion cells (RGCs) from their retinorecipient areas or the optic nerve (Salinas-Navarro et al., 2010; Nadal-Nicolas et al., 2015).
Retrograde tracing with Fluoro-Gold: Different methods of …
https://www.researchgate.net/publication/223454644_Retrograde_tracing_with_Fluoro-Gold_Different_methods_of_tracer_detection_at_the_ultrastructural_level_and_neurodegenerative_changes_of_back-filled_neurons_in_long-term_studies
among the available retrograde fluorescent tracers fluoro-gold (fg) is particularly advantageous because it (1) is not only detectable by fluorescence microscopy but also immunocytochemically,...
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