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Retrograde Tracing - an overview | ScienceDirect Topics
https://www.sciencedirect.com/topics/neuroscience/retrograde-tracing
The anterograde tracing data confirm the retrograde tracing results, demonstrating that the iontophoretic injections of PHA-L indeed label neurons in R that give origin to axons crossing the midline and terminating in the contralateral reticular, anterior, and intralaminar nuclei.
BAcTrace, a tool for retrograde tracing of neuronal ...
https://www.nature.com/articles/s41592-020-00989-1
Here we present BAcTrace (Botulinum-Activated Tracer), a genetically encoded, retrograde, transsynaptic labeling system. BAcTrace is based on Clostridium botulinum neurotoxin A, Botox, which we...
Monosynaptic retrograde tracing of neurons expressing …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5601234/
Retrograde Cre‐dependent tracing using pseudotyped rabies virus Monosynaptic tracing of the afferents of Cre‐expressing neurons (Callaway & Luo, 2015; Watabe‐Uchida et al., 2012) was carried out in two steps.
BAcTrace, a tool for retrograde tracing of neuronal ...
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7610425/
Here we present BAcTrace (Botulinum Activated Tracer), a genetically encoded, retro-grade, transsynaptic labelling system. BAcTrace is based on C. botulinumneurotoxin A, Botox, which we have engineered to travel retrogradely between neurons to activate an otherwise silent transcription factor.
Monosynaptic retrograde tracing of neurons expressing …
https://pubmed.ncbi.nlm.nih.gov/28657115/
The Gpr151-expressing neurons in the paraventricular nucleus of the thalamus was primarily contacted by medial hypothalamic areas as well as the zona incerta and projected to specific forebrain areas such as the prelimbic cortex and the accumbens nucleus.
BAcTrace, a tool for retrograde tracing of neuronal ...
https://pubmed.ncbi.nlm.nih.gov/33139893/
BAcTrace, a tool for retrograde tracing of neuronal circuits in Drosophila Animal behavior is encoded in neuronal circuits in the brain. To elucidate the function of these circuits, it is necessary to identify, record from and manipulate networks of connected neurons.
Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
Retrograde tracing is a research method used in neuroscience to trace neural connections from their point of termination (the synapse) to their source (the cell body ). Retrograde tracing techniques allow for detailed assessment of neuronal connections between a target population of neurons and their inputs throughout the nervous system.
Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
Fluoro-Gold (FG) is a potent water-soluble crystalline retrograde neuronal tracer with following properties: strong fluorescence, extensive filling of neurons, labeling neuronal soma and dendrites, high resistance to bleaching, no uptake by intact undamaged fibers of passage, no diffusion from labeled cells; and not transported transsynaptically.
Frontiers | A Student’s Guide to Neural Circuit Tracing ...
https://www.frontiersin.org/articles/10.3389/fnins.2019.00897/full
Tracers are categorized as anterograde or retrograde based on their direction of travel within neurons. Anterograde tracers (green) are taken up by neuronal cell bodies at the injection site and travel down the axon to terminal processes. Retrograde tracers (blue) are taken up by terminals and travel back to the cell body.
Using AAV for neuronal tracing - Addgene
https://blog.addgene.org/using-aav-for-neuronal-tracing
Anterograde tracing outlines neurons from their cell bodies to the terminals of their axons; while retrograde tracing outlines neurons in the opposite direction, from the terminals of their axons to their cell bodies. Anterograde and retrograde tracing take advantage of existing transport pathways in neurons.
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