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Viral neuronal tracing - Wikipedia
https://en.wikipedia.org/wiki/Viral_Neuronal_Tracing#:~:text=Retrograde%20tracing%20is%20the%20use%20of%20a%20tracer,kinesin%2C%20and%20so%20will%20spread%20at%20different%20rates.
Retrograde tracing - Wikipedia
https://en.wikipedia.org/wiki/Retrograde_tracing
Anterograde or Retrograde Transsynaptic Circuit Tracing …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4776322/
To PDL-treat plates, add 10% PDL to tissue culture–grade water, and add to dish to coat the surface of the plate. Rock gently to ensure even coating. After 5 min, aspirate solution and rinse with sterile tissue culture-grade water. Allow 2 hr for the plates to dry. 2
Anterograde or Retrograde Transsynaptic Circuit Tracing …
https://pubmed.ncbi.nlm.nih.gov/26729030/
Abstract Viruses have been used as transsynaptic tracers, allowing one to map the inputs and outputs of neuronal populations, due to their ability to replicate in neurons and transmit in vivo only across synaptically connected cells. To date, their use …
Anterograde or Retrograde Transsynaptic Circuit Tracing …
https://www.ncbi.nlm.nih.gov/sites/ppmc/articles/PMC4776322/
To PDL-treat plates, add 10% PDL to tissue culture–grade water, and add to dish to coat the surface of the plate. Rock gently to ensure even coating. After 5 min, aspirate solution and rinse with sterile tissue culture-grade water. Allow 2 hr for the plates to dry. 2
Retrograde Tracing - labome.com
https://www.labome.com/method/Retrograde-Tracing.html
Retrograde transport requires an uptake of a tracer along the axons projecting from the infusion site and transport to a distal area where it …
Anterograde or retrograde transsynaptic labeling of CNS …
https://pubmed.ncbi.nlm.nih.gov/21825165/
Anterograde or retrograde transsynaptic labeling of CNS neurons with vesicular stomatitis virus vectors Abstract To understand how the nervous system processes information, a map of the connections among neurons would be of great benefit. Here we describe the use of vesicular stomatitis virus (VSV) for tracing neuronal connections in vivo.
Untangling Neural Networks with Dual Retrograde …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2796918/
Using recombinant rabies virus (RV), we developed a dual transsynaptic retrograde tracing technique in the rat central nervous system. Two strains of recombinant RV, injected into two separate loci of the brain, were taken up through axon terminals and carried retrogradely and transsynaptically from neuron to neuron.
BAcTrace, a tool for retrograde tracing of neuronal …
https://www.nature.com/articles/s41592-020-00989-1
Here we present BAcTrace (Botulinum-Activated Tracer), a genetically encoded, retrograde, transsynaptic labeling system. BAcTrace is based on Clostridium botulinum neurotoxin A, Botox, which we...
AAV-Mediated Anterograde Transsynaptic Tagging: …
https://pubmed.ncbi.nlm.nih.gov/27989459/
Despite the successful application of retrograde transsynaptic viruses for identifying presynaptic neurons of transduced neurons, analogous anterograde transsynaptic tools for tagging postsynaptically targeted neurons remain under development.
A Cre-dependent, anterograde transsynaptic viral tracer …
https://pubmed.ncbi.nlm.nih.gov/22196330/
Genetically targetable retrograde transsynaptic tracer viruses are available to map the inputs to specific neuronal subpopulations, but an analogous tool for mapping synaptic outputs is not yet available. Here we describe a Cre recombinase-dependent, anterograde transneuronal tracer, based on the H129 strain of herpes simplex virus (HSV).
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