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(PDF) Tracing Microtubules in Live Cell Images.
https://www.researchgate.net/publication/221623236_Tracing_Microtubules_in_Live_Cell_Images
High resolution images capable of distinguishing individual microtubules and at the same time containing an entire cell in the field of view are difficult to obtain, and therefore researchers ...
TRACING MICROTUBULES IN LIVE CELL IMAGES
https://scl.ece.ucsb.edu/sites/scl.ece.ucsb.edu/files/publications/f07_1_0.pdf
TRACING MICROTUBULES IN LIVE CELL IMAGES M. E. Sarg õn1,A.Altõnok 1,E.Kiris2,S.C.Feinstein2,L.Wilson2,K.Rose1, B. S. Manjunath 1 ... tracking of MTs in live cell images. Aside of very low signal to noise ratios, live cell images of MTs exhibit severe clut-ter for accurate tracing of MT body. Moreover, intersecting
CiteSeerX — TRACING MICROTUBULES IN LIVE CELL …
https://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.79.8258
Recently, computer vision techniques have been applied to the problem of automated tracking of MTs in live cell images. Aside of very low signal to noise ratios, live cell images of MTs exhibit severe clutter for accurate tracing of MT body. Moreover, intersecting and overlapping MT regions appear brighter due to additive fluorescence.
TRACING MICROTUBULES IN LIVE CELL IMAGES - INFONA
https://www.infona.pl/resource/bwmeta1.element.ieee-art-000004193281
Microtubule (MT) dynamics are traditionally analyzed from time lapse images by manual techniques that are laborious, approximate and often limited. Recently, computer vision techniques have been applied to the problem of automated tracking of MTs in live cell images. Aside of very low signal to noise ratios, live cell images of MTs exhibit severe clutter for …
Tracking Microtubules in Live Cells | Imaris- Oxford …
https://imaris.oxinst.com/learning/view/article/tracking-microtubules-in-live-cells-with-high-spatial-resolution
Wu’s research team used dual-view plane illumination microscopy and Imaris to study microtubule assembly dynamics in 3D by acquiring time-lapse images of whole-cell volumes of human umbilical vein endothelial cells. The cells …
Tracing Microtubules In Live Cell Images
https://web.ece.ucsb.edu/~manj/Munich/(Munich)Microtubule.ppt
Tracing Microtubules In Live Cell Images Author: msargin Last modified by: msargin Created Date: 12/9/2006 4:26:47 AM Document presentation format: On-screen Show Company: UCSB Other titles: Arial Default Design Adobe Acrobat 7.0 Document Microsoft Equation 3.0 Motivation Challenges Tip Detection Methodology Results ...
Visualize Microtubule Dynamics in Live Cells | Thermo …
https://www.thermofisher.com/us/en/home/references/newsletters-and-journals/bioprobes-journal-of-cell-biology-applications/bioprobes-79/tubulin-tracker-deep-red-microtubule-dynamics-live-cells.html
Invitrogen Tubulin Tracker Green has long been used to visualize microtubules in live cells. However, its short-wavelength excitation produces rapid photobleaching of the signal ( Figure 1B) and increases phototoxicity to the cells during extended time-lapse imaging. Additionally, Tubulin Tracker Green cannot be multiplexed with commonly used ...
Live cell-imaging techniques for analyses of microtubules …
https://pubmed.ncbi.nlm.nih.gov/20719279/
Dictyostelium amoebae provide a popular model system for analyses of cell and cytoskeletal dynamics. Yet, the sensitivity of Dictyostelium cells to phototoxic effects, their rapid cell movement, and the extraordinary motility of their microtubule system are specific challenges for live cell imaging. The protocols outlined in this chapter are ...
BioProbes article: Visualize microtubule dynamics in …
https://assets.thermofisher.com/TFS-Assets/BID/Reference-Materials/tubulin-tracker-deep-red-microtubule-dynamics-live-cells-bioprobes-79-article.pdf
that selectively stains microtubules in live cells and exhibits deep-red emission. Eliminating the need for genetic modification, Tubulin Tracker Deep Red provides uniform labeling of the microtubule cytoskeleton in live cells with a rapid and simple staining protocol. The far-red spectral properties of Tubulin Tracker Deep Red—with
A generative model of microtubule distributions, and …
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2901542/
In order to build a model from a 3D cell image, the nuclear and cell membrane channels were deconvolved with their respective point spread functions and segmented semi-automatically using the Active Contour without Edges approach (see Methods). ... Tracing Microtubules in Live Cell Images. 2007. pp. 296–299. 21. Ponti A, Matov A, Adams M ...
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